The molecular epidemiology of Staphylococcus aureus of bovine mastitis origin
نویسندگان
چکیده
The present study was aimed to understand the molecular epidemiology of Staphylococcus aureus (54 isolates), isolated from 422 milk samples obtained 108 subclinical mastitis affected cows (CMT positive ≥1+ in at least one quarter). studied using coagulase (coa) gene polymorphism, 16S-23S ribosomal spascer (RS-PCR) polymorphism and Staphylococcal protein A (Spa) typing. produced 7 genotypes 8 RS respectively. Coagulase genotype GTIII (730 bp) most prevalent (35 strains) followed by GTV (900 bp, GTIV (800 4 strains), whereas GTA accounted for highest number strains (31 GTB (11strains), GTH (4 GTE (3 strains). CTIII showed diversity, as isolates within it 5 genotypes, majority them belonging (29 out 31 Forty 54 this were correctly typed spa typing, assigned 21 known types, new novel type t18462. revealed high diversity strains, consisting 22 types.
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ژورنال
عنوان ژورنال: Veterinarski Arhiv
سال: 2021
ISSN: ['0372-5480', '1331-8055']
DOI: https://doi.org/10.24099/vet.arhiv.0808